The save directory changes slightly depending on your operating system:

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Once you have downloaded a file named something like S3AIR_101.sav or slot_1.sav , follow these steps:

If you meant something else by "101 save file" (e.g., a specific mod, a bug report, or a 101% completion save), let me know and I can refine the answer further.

Clear specific zones under strict time limits that mimic competitive speedrun routes. 3. Alternative Character Runs